摘要
FD－TRT是一种耐热逆转录酶（是自行表达并提纯的），根据TapDNA聚合酶的晶体结构为模板，采用计算机同源模建的方法建立FD－TRT的三级结构模型，并对FD－TRT和DNA聚合酶家族的其他蛋白功能差异的结构基础以及保守性功能性残基的作用进行研究，从而为进一步改造FD－TRT分子、提高其耐热性、反应活性以及对RNA模板和DNA模板的选择性提供良好的基础．
In this paper, by comparing three automatic homology modeling medhods and taking the crystal structure of Taq polymerase as model block, the authors adopt a combined method to build a tertiary structure model of FD-thermostable reverse transcriptase (FDTRT), which was constructed and expressed by their laboratory earlier. The model makes them possible to investigate the structure basis for the functional difference between FDTRT and other proteins in DNA polymerase family. Furthermore, they also study the role of the reserved functional residue on the tertiary structure level. Thus some clues have been revealed for the further improvement of FD-TRT, such as raising its thermostability and reaction activity, offering good basis for the selectivity of RNA model block and DNA model block.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期455-461,共7页
Journal of Fudan University：Natural Science
基金
国家自然科学基金
关键词
三级结构
同源建模
嗜热细菌
耐热性
逆转录酶
tertiary structure
homology modeling
thermostability
reverse transciptase
