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Rhodobacter sphaeroides偶氮还原酶三级结构同源建模与分析 被引量：3

Homology modeling and analysis of three-dimensional structure of azoreductase of Rhodobacter sphaeroides
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摘要 利用同源建模方法构建了Rhodobacter sphaeroides偶氮还原酶AZR的三级结构模型.结果表明,AZR为α/β型结构的黄素氧化还原蛋白,5个相互平行的β折叠形成分子中间的平面,5个α螺旋分列于平面的两侧;在β折叠的C端非共价结合的黄素单核苷酸(FMN)作为氧化还原反应中心.根据序列对齐分析,将依赖黄素的偶氮还原酶分为两个家族,但结构对比分析表明,它们具有类似的三级结构和活性区域.对AZR的结构研究为发现新的偶氮还原酶和深入研究其功能奠定了基础. The three-dimensional structure model of azoreductase AZR of Rhodobacter sphaeroides was constructed using homology modeling method. It is demonstrated that AZR is a flavodoxin adopting α/β structure with the central five-stranded parallel β-sheet flanked by five α helices. A flavin mononucleotide （FMN） prosthetic group lies on top of the molecule,acting as redox and reactive centers. Flavin-dependent azoreductases are classified into two families according to the results of sequence alignments. However,structure analysis indicates that they all possess similar three-dimensional structures and active sites. The study of AZR＇s structure lays a foundation for finding new azoreductase gene and deeply understanding AZR＇s function.
作者 柳广飞 周集体 王竞 周觅
机构地区 大连理工大学环境学院
出处 《大连理工大学学报》 EI CAS CSCD 北大核心 2010年第2期171-175,共5页 Journal of Dalian University of Technology
关键词 偶氮还原酶 同源建模 黄素氧化还原蛋白 azoreductase homology modeling flavodoxin
分类号 Q518.2 [生物学—生物化学]
  • 相关文献

参考文献9

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共引文献24

同被引文献34

  • 1姜国建,于仁诚,王云峰,颜天,周名江.中国明对虾(Fenneropenaeus chinensis)血细胞中一氧化氮合成酶的鉴定及其在白斑综合症病毒感染过程中的变化[J].海洋与湖沼,2004,35(4):342-350. 被引量：24
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引证文献3

二级引证文献7

大连理工大学学报
2010年 第2期

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