摘要
目的 为检测丙型肝炎病毒，建立 Ｆ Ｄ 耐热逆转录酶介导的逆转录ｎｅｓｔｅｄ Ｐ Ｃ Ｒ 系统。方法 用该系统对１０８ 例可疑患者的血液进行检测，并与 Ｅ Ｌ Ｉ Ｓ Ａ 法的结果进行比较。结果 实验表明， Ｆ Ｄ 耐热逆转录酶介导的逆转录ｎｅｓｔｅｄ Ｐ Ｃ Ｒ 比 Ｅ Ｌ Ｉ Ｓ Ａ 检出了更高的阳性率。结论 该系统是一种灵敏、有效、特异的 Ｈ Ｃ Ｖ 检测方法。
Detection for HCV with FDthermostable reverse transcriptase mediated RTnested PCR$$$$ ZHENG Zuohua, ZHANG Liping, ZHAO Wei, et al. Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433 【 Abstract 】 Objective In order to detect HCV in human blood, a FDthermostable reverse transcriptase mediated RTnested PCR technique was developed. Methods For comparison, we used this method and conventional ELISA to detect the infection of HCV in 108 patients. Results The results showed higher positive rate in FDTRT mediated RTnested PCR. Conclusion The results suggest that it is a more sensitive, effective and specific way for detecting HCV infection. 【 Subject words 】 FDthermostable reverse transcriptase Polymerase chain reaction Hepatitis C virus
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1999年第5期445-447,共3页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
上海市自然科学基金
