摘要
目的:观察胃黏膜细胞c-Jun氨基末端激酶(JNK)在室旁核(PVN)内微量注射催产素(OT)对大鼠胃缺血-再灌注(GI-R)损伤调控中的作用及机制.方法:将SD大鼠随机分为4组:vehicle组,OT组,OT+atosiban组,atosiban组.采用夹闭大鼠腹腔动脉30min,去除动脉夹再灌注1h的GI-R损伤模型,于单侧PVN微量注射OT.运用免疫组织化学及免疫印迹等实验技术,观察了PVN微量注射OT对GI-R后胃黏膜细胞p-JNK、Bax和Bcl-2蛋白表达的影响.结果:与vehicle组相比,PVN内微量注射OT(600ng)能明显减少GI-R后胃黏膜细胞p-JNK及Bax的表达(均P<0.01),增加Bcl-2的表达(P<0.01).侧脑室给予OT受体特异性拮抗剂atosiban后,可取消OT的效应,与OT组比较,差异有统计学意义(F=56.33,P<0.01;F=145.2,P<0.01,F=49.32,P<0.01),胃黏膜细胞p-JNK及Bax的表达较OT组明显增加,而Bcl-2的表达减少.结论:PVN微量注射OT减轻GI-R损伤的调控机制,在胃黏膜局部是通过抑制胃黏膜细胞p-JNK和Bax的表达,促进Bcl-2的表达实现的.
AIM： To investigate the molecular mechanism underlying the role of JNK in mediating the effects of oxytocin （OT） microinjected into the paraventricular nucleus （PVN） on gastric ischemia-reperfusion （GI-R） injury. METHODS： Sprague-Dawley （SD） rats were randomly divided into four groups： vehicle group, OT group, atosiban group and OT plus atosiban group. GI-R injury was induced in rats by clamping the celiac artery for 30 min and then reperfusing for I h. A cannula was inserted into the unilateral PVN for microinjection of OT. The expression of p-JNK, Bax and Bcl-2 proteins in rat gastric mucosa was examined by Western blot and immunohistochemistry. RESULTS： Compared with the vehicle group, microinjection of OT （600 ng） into PVN signifi- cantly decreased the expression of p-JNK and Bax proteins but increased the expression of Bcl-2 protein in gastric mucosa following GI-R （all P 〈 0.01）. Pre-administration of atosiban （an OT receptor antagonist） into the lateral cerebral ventricle could prevent the effects of OT （F=56.33, P 〈 0.01; F = 145.2, P 〈 0.01, F = 49.32, P〈 0.01）, increase the expression of p-JNK and Bax proteins, and decrease the expression of Bcl-2 protein when compared with the OT group. CONCLUSION： Microinjection of OT into PVN attenuates GI-R injury through down-regulation of p-JNK protein, which in turn leads to a decrease in Bax expression and an increase in Bcl-2 expression.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第19期1919-1924,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30570671
江苏省脑病生物信息重点实验室开放基金资助项目
JSBL0804~~
关键词
胃缺血-再灌注
室旁核
催产素
P-JNK
Gastric ischemia-reperfusion
Paraventricular nucleus
Oxytocin
p-JNK
