摘要
采用骨髓有核细胞微核率测定法，检测小鼠服用鲤鱼精巢ＤＮＡ后，６０Ｃｏ－γ射线对小鼠骨髓细胞染色体损伤的严重程度，结果表明，小鼠每天每千克体质量灌胃鲤鱼精巢ＤＮＡ１００ｍｇ，连续１５ｄ，可使总剂量６Ｇｙ的６０Ｃｏ－γ射线致小鼠骨髓有核细胞微核发生率减少４７．２％（Ｐ＜０．００１）；采用全血ＵＤＳ测定法，检测小鼠血细胞ＤＮＡ受紫外线损伤后的修复速度，结果表明，小鼠每天每千克体质量灌胃鲤鱼精巢ＤＮＡ３０ｍｇ，连续３个月，其全血ＵＤＳ增加４０．０２％（Ｐ＜０．００１）．
60Co-gamma ray can injure the chromesomes of bone marrow cells, and this injury may be measured by determining the rate of micronuclei of bone marrow cells of irradiated mice. Our observations indicated that the formation rate of micronuclei of bone marrow cells could decrease 472% if the mice had administered DNA from carp spermary (100 mg/kg weight) for 15 days before they were irradiated. Ultroviolet can damage DNA of cells. The damaged DNA can be repaired by the repair enzymes system in cells. The repairing speed can be measured by 3H-TdR intercalation (ie unscheduled DNA synthesis measurement, UDS measurement ). The results showed that the UDS of whole blood cells in mice had increased 4002% after DNA from carp spermary (30 mg/kg weight ) had been administered to the mice for 3 months.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期125-127,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
关键词
鲤鱼
精巢
微核率
DNA
外源性
carp, spermary, deoxyribonucleic acid(DNA), rate of micronuclei, unscheduled DNA synthesis(UDS)
