摘要
采用反相高效液相色谱法，同时测定食品中常见的10种食品添加剂：苯甲酸、山梨酸、对羟基苯甲酸甲酯（尼泊金甲酯）、糖精钠、安赛蜜、柠檬黄、日落黄、胭脂红、苋菜红、亮蓝。实验采用Symmetry C18柱（4．6mm×150mm，5μm，Waters）为分离柱，以20mmol／L乙酸铵（pH6．8）-甲醇为流动相，用紫外检测器在234nm下进行检测，整个分析过程在17min内完成。其平均加标回收率为94．14％～99．94％，相对标准偏差小于4％。该方法结果准确，适用于食品常规质量检测。
A novel method is proposed for the simultaneous separation and determination of ten food additives （benzoic acid, sorbic acid, methyl p-hydroxybenzoate, saccharin sodium, acesulfame potassium, tartrazine, sunset yellow, ponceau 4R, amaranth, brilliant blue） by reversed-phase high performance liquid chromatography. The separation was achieved within 17 min by using a Symmetry reverse phase C18 colum （150 mm ×4.6 mm, 5μm, Waters Inc） with 20 mmol/L NH4Ac （pH6.8）-methanol as mobile phase and lastly detected at 234 nm through a UV detector. The average recoveries for real samples ranged from 94.14% to 99.94%. The relative standard deviation was less than 4%, This method is accurate and applicable to conventional food quality testing.
出处
《食品科技》
CAS
北大核心
2009年第8期275-279,共5页
Food Science and Technology
关键词
食品
食品添加剂
高效液相色谱法
food
food additives
high performance liquid chromatographys
