摘要
为了探讨全反式维甲酸（ＡＴＲＡ）治疗大肠癌的机制，以大肠癌细胞株ＬｏＶｏ为研究对象，用ＭＴＴ、ＦＣＭ、ＴＤＴ、电泳和电镜等手段，观察ＡＴＲＡ对ＬｏＶｏ细胞的作用，结果显示细胞增殖受到抑制，高浓度组ＦＣＭ出现凋亡峰，电镜下有大量凋亡细胞，细胞凋亡比例增高。可以认为ＡＴＲＡ能诱导ＬｏＶｏ细胞凋亡。
In order to illustrate the possible mechanisms of ATRA in treatment of colorectal neoplasm, the colonic cancer cell line LoVo was used for studies in vitro. The effects of ATRA on LoVo were measured by MTT, flow cytometry, DNA electrophonesis, TDT and electronic microscopy. MTT study showed significantly inhibited proliferation. An extra 'subGO/G1' peak appeared in higher dose of ATRA group by flow cytometric study. Apoptosis cells were found by electronic microscopy. The increase of percentage of apoptosis cells was assayed by TDT.These observations imply that ATRA can induce apoptosis of LoVo cell definitely.
出处
《实用癌症杂志》
1998年第3期167-170,共4页
The Practical Journal of Cancer
