摘要
目的：提取并纯化豚鼠脑、脊髓髓鞘碱性蛋白（ＭＢＰ），对其理化特征和致实验性自身免疫性脑脊髓炎（ＥＡＥ）的活性进行鉴定，并初步探讨口服ＭＢＰ诱导免疫耐受防治ＥＡＥ的作用。方法：以豚鼠脑、脊髓为原料，通过去脂、抽洗、酸溶解、高速离心等步骤提取ＭＢＰ，并在Ｗｉｓｔａｒ大鼠中诱导ＥＡＥ和口服耐受。结果：所提取的ＭＢＰ具有与文献报告结果类似的理化特征和诱发ＥＡＥ的活性；通过口服ＭＢＰ，成功诱导出对ＥＡＥ的免疫耐受。结论：ＭＢＰ对研究ＥＡＥ的发病机制和病理变化及通过口服ＭＢＰ诱导免疫耐受对ＥＡＥ和多发性硬化症的防治具有重要意义。
ABSTRACT Objective: To study physical and chemical characteristics and biological activity of myelin basic protein(MBP) from guinea pig brain and spinal cord. To investigate the activity of MBP in inducing experimental autoimmune encephalomyelitis(EAE) and suppression of EAE by oral administration of MBP. Methods: By means of defatting with methanol-chloroform, acid extraction and centrifugation , MBP was obtained . MBP was emulsified in complete Freund's adjuvant(CFA)and was used to induce EAE and oral tolerance in Wistar rats. Results: EAE was successfully induced with MBP plus adjuvant in Wistar rats. In addition, MBP was found to have protective action and therapeutic effect on EAE when animals were fed MBP before or after disease induction. Conclusion: MBP can be used to induce immunologic tolerance in the prevention and treatment of EAE.
出处
《北京医科大学学报》
CSCD
1998年第4期320-322,共3页
Journal of Peking University(Health Sciences)
基金
高等学校博士学科点专项科研基金
