摘要
本文介绍了从人脑中分离纯化髓鞘碱性蛋白的方法,人脑组织匀浆经甲醇—氯仿脱脂、酸提取、硫酸铵沉淀和羧甲基纤维素柱层析,得到了纯化的髓鞘碱性蛋白。该蛋白在SDS聚丙烯酰胺凝胶电泳中为单一带,分子量为21kD。在聚焦电泳中测得其等电点在pH10以上,氨基酸组成分析结果也与文献值接近。这为进一步研究人脑髓鞘碱性蛋白的抗原性创造了条件。
A Procedure was developed for purification of myelin basic protein (MBP) from human brain. The purified MBP was obtained by means of defatting with me thanol-chloroform of human brain homogenate, acid extraction, ammonium sulfate precipitation and chromatography on column of CM-cellulose. The MBP purified by this procedure appears homogeneous on SDS-PAGE. The apparent molecular weight is 21000Da,The pI value is above pH10 on isoelectric focusing gel.The amino acid com positon appears indistinguishable from that reported previously in reference.
基金
国家自然科学基金项目3870202号资助
关键词
髓鞘碱性蛋白
脑
分离
纯化
Myelin basic protein, SDS-PAGE
Amino acid composition analysis
