摘要
分别克隆番茄PR-1和PR-5基因片段,并以之制备探针,采用Northern blot方法研究了PR-1和PR-5基因在正常生长条件下番茄根、茎、叶、果实发育中的表达模式,研究了干旱、复水和内外源乙烯对这两种基因表达的影响.结果表明:这两个基因主要在衰老叶片、B期果实和根部表达,干旱抑制了这两个基因的表达,复水和乙烯诱导其表达.
PR-1 and PR-5 gene fragments were isolated with PCR and RT-PCR from the genomic DNA and eDNA of tomato leaves. Then the specific DNA fragments of PR-1 and PR-5 genes were used as probes to hybridize with the total RNAs extracted from the wild type tomato （AC^＋＋） and the ethylene response mutants of tomato （Nr, T4B-11） to analyse the expression pattern of the two genes in tomato root, stem, leaf and fruit in normal conditions and to study the effect of drought, re-watering and exogenous and endogenous ethylene on the expression of the two genes. Northern blot hybridization results indicated that PR-1 and PR-5 genes were expressed mainly in senescent leaves, fruit at Stage B and roots,and thheir expression was restrained by drought and induced by re-watering and ethylene.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第8期67-72,共6页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金资助项目(30600044)
重庆市自然科学基金资助项目(2006BB1194
2007BB1197)
