摘要
目的:建立同时测定黄芪药材中毛蕊异黄酮葡萄糖苷和黄芪甲苷的方法。方法:采用HPLC仪,色谱柱Waterssymmetry C18(4.6 mm×250 mm,5μm),流动相乙腈-水(30∶70),体积流量1 mL.min-1,柱温35℃,检测波长260 nm(毛蕊异黄酮葡萄糖苷),210 nm(黄芪甲苷)。结果:毛蕊异黄酮葡萄糖苷,黄芪甲苷线性范围分别为0.391～9.78,0.974～24.3μg,平均加样回收率分别为99.18%(RSD 2.43%),99.41%(RSD 2.59%)。结论:该方法简单快捷,可用于黄芪药材中毛蕊异黄酮葡萄糖苷和黄芪甲苷的同时测定,为含黄芪制剂质量标准的建立提供了参考。
Objective： To establish a method for simultaneously determining calycosin-7-O-β-D-glucoside and astragaloside Ⅳ in Astragali Radix by HPLC. Method： HPLC was performed on Waters symmetry C18 column （4.6 mm×250 mm, 5 μm）, the mobile phase consisted of 30% acetonitrile and 70% water at a flow rate of 1 mL·min^-1 The column temperature was set at 35 ℃, and the eluate was detected at 260 nm and 210 nm. Result： Quantitative analysis of HPLC showed that the linear ranges of calycosin-7-O-β-D-glucoside and astragaloside Ⅳ were 0.391 2-9.78, 0.973 6-24.34 μg respectively. The average recoveries of two ingredients were 99.18% （RSD 2.43%） and 99.41% （RSD 2.59%）. Conclusion： The method set up is simple and rapid, therefore available for the quality control of calycosin-7-O-β-D-glucoside and astragaloside Ⅳ in Astragali Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期115-117,共3页
Chinese Journal of Experimental Traditional Medical Formulae
