摘要
目的制备长双歧杆菌和嗜酸乳杆菌二联活菌微胶囊，以期提高口服时的存活率和常温下的保存期。方法根据微胶囊在人工胃液中的耐酸性和在人工肠液中的崩解性，确定空气悬浮法制备微胶囊的最佳工艺条件。扫描电镜观察微胶囊形态与大小。根据恒温37℃、相对湿度60％～65％条件下贮存3个月后菌体存活率，考察其贮存稳定性。结果制备的微胶囊具有良好的耐酸性和肠溶性。微胶囊包囊产率56．49％，包囊效率87．45％。微胶囊表面覆盖着一层光滑平整的囊膜，粒径大小基本在400～500μm。加保护剂的微胶囊菌体存活率高于10％，且远远高于冻干菌粉和无保护剂微胶囊组。结论确定了空气悬浮法制备长双歧杆菌和嗜酸乳杆菌二联活菌微胶囊的最佳工艺条件，微胶囊化有利于提高活菌的稳定性。
Objective To prepare the microcapsule containing Bifidobacterium and Lactobacillus acidophilus in order to improve the oral survival rate and shelf life at room temperature. Methods According to the acid-resistance in artificial gastric juice and collapse in artificial intestinal juice of microcapsule, the optimal technological conditions were ascertained for the preparation of microcapsule with air suspension. The shape and size of microcapsule were observed with scanning electron microscope. The storage stability of bacteria was studied according to the survival rate of microencapsulated bacteria after storage for 3 months at 37 ℃and RH 60%-65%. Results Bacteria trapped in the microcapsule survived well in artificial gastric juice and collapsed easily in artificial intestinal iuice, The encapsulation yield of microcapsule was 56.49 %, and the encapsulation efficiency was 87.45 %. The surface of microcapsule was covered with a smooth film. The particle diameter was 400-500 μm. The survival rate of mieroencapsulated bacteria in the presence of protectants after storage for 3 months at 37 ℃ and RH 60% -65% was over 10 % ,which was higher than that of lyophilized powder and microencapsulated bacteria in the absence of protectants. Conclusion The optimal technological conditions can be obtained for the preparation of microcapsule containing Bifidobacterium and Lactobacillus acidophilus with air suspension. The stability of microenc ＇,apsulated bacteria can be improved.
出处
《食品与药品》
CAS
2009年第4期11-16,共6页
Food and Drug
关键词
双歧杆菌
嗜酸乳杆菌
微胶囊
Bifidobacterium
Lactobacillus acidophilus
microcapsule
