摘要
用62个大豆DNA分子探针与5种限制性内切酶组合,对大豆基因组DNA限制性片段长度多态性(RFLP)进行分析。结果表明,所选用的两对材料,其多态性RFLP标记的频率均高达60％,可作为RFLP作图较理想的亲本;RFLP标记的多态性类型多数表现为共显性,少数为显性,其中一些标记揭示了2个或2个以上的独立分离的基因座位;不同限制性内切酶在揭示多态性的能力上差异不显著,在杂交片段长度上差异显著并都大于预期值;大豆RFLP的形成主要是由DNA的重排所引起。
Studies were conducted to evaluate the RFLP diversity in soybean with sixty-
two RFLP probes and five restriction enzymes. Two pairs of accessions were found to be ideal
parents for mapping and both of them had sixty percent of the polymorphic markers. Most
RFLP markers were codominant and some were dominant. In a few cases, a single RFLP probe
revealed two or more polymorphic locus. Among enzymes, there were no significant difference
for detecting polymorphisms, but the mean observed fragment size varied significantly and were
larger than expected for all enzymes. DNA rearrangement was the main reason for soybean
RFLP diversity.
出处
《作物学报》
CAS
CSCD
北大核心
1998年第4期486-490,共5页
Acta Agronomica Sinica
基金
中国科学院重大项目基金资助
关键词
多态性
大豆
品种间
DNA
RFLP
Restriction fragment length polymorphism(RFLP)
Soybean
