摘要
分子标记连锁图的构建为植物基因组的结构和功能分析提供了有力的工具。较高密度的遗传图谱在数量性状基因定位、图位克隆重要农艺性状基因等研究中发挥了巨大作用。应用栽培大豆长农４和半野生大豆新民６杂交得到的Ｆ８代重组自交系，构建了一张较高密度的遗传图谱。该图谱共有２４０个标记，其中包括２个形态标记、１００个ＲＦＬＰ标记、３３个ＳＳＲ标记、４２个ＡＦＬＰ标记、６２个ＲＡＰＤ标记和１个ＳＣＡＲ标记，分布在２２个连锁群上，总长度为３ ７１３．５ｃＭ，覆盖了整个基因组。对 ７２个 ＲＦＬＰ探针的分析表明，其中有 １６个能揭示 ２个或 ２个以上独立分离的遗传位点；说明大豆基因组中存在广泛的同源区域。结果表明本图谱与大豆公共图谱有较好的可比性，可进行一般的ＱＴＬ分析。
Molecular linkage maps provide a powerful tool for the analysis of plant genome structure and function including the detection of quantitative trait loci (QTL). The molecular linkage map of soybean was constructed using an 88-individual RIL population derived from a cross between cultivated Changnong 4 and semi-wild Xinmin 6. The map contains 22 linkage groups and 240 markers, including 100 RFLP, 33 SSR, 62 RAPD, 42 AFLP, 1 SCAR and 2 morphological markers. It covers approximately 3 713.5 cM of soybean genome. Analysis of 72 RFLP markers showed that 16 of them revealed two or more independent loci and it suggested that there is an extensive homologous region in soybean genome. This map compared very well with the other published soybean maps.
基金
国家高技术计划!(863-101-02-02-03)
国家重点基础项目!(G1998010208)资助
