摘要
为揭示玉米胞质雄性不育的生化机制,以玉米C型雄性不育系C478及其保持系478、恢复系H01为材料,对叶片及雄穗小花的可溶性糖、可溶性蛋白、游离脯氨酸含量进行比较研究.结果表明:玉米不育系苗期、抽雄期、开花期叶片中可溶性糖含量均显著高于保持系、恢复系;三系间可溶性蛋白含量相差小;不育系除抽雄期外叶片中的游离脯氨酸含量显著高于其保持系、恢复系.玉米雄穗发育过程中,保持系雄穗小花中的可溶性糖含量在雄穗孕育完全但没抽出期(时期Ⅱ)、散粉期(时期Ⅲ)显著高于不育系;不育系小花中各个时期的可溶性蛋白含量均低于其保持系和恢复系,特别在时期Ⅱ、时期Ⅲ显著低于保持系和恢复系;不育系每个时期小花中的游离脯氨酸含量都显著低于保持系和恢复系.
In order to make further study on the biochemical mechanism of C-cytoplasmic male sterility of maize, C478, 478 and H01, the cytoplasmic male＇ sterility line and its maintainer and restorer line of maize were used as the raw material to comparatively analyze the contents of soluble sugar, soluble protein and free proline in their tassel florets. The results showed that soluble sugar content in the male sterility line was significantly higher than its maintainer and restorer in every stages except the elongation of leaves. The soluble protein contents had little difference among three lines. Free proline content in the male sterility line was significantly higher than its maintainer and restorer line in every stage except the tasseling of leaves. As the tassel developed, soluble sugar in the maintainer line was significantly higher than the male sterility line in stage Ⅱ and stage Ⅲ. Soluble protein content in the male strerility line was lower than that of its maintainer and restorer line in every stage of florets development, and the difference was obvious in stage Ⅲ and stage Ⅲ. Free proline content in the male sterility line was significantly lower than that of its maintainer and restorer line in every stage of florets development.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第3期249-251,共3页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科技支撑计划项目(2008NK2002)
湖南农业大学生物科学技术学院大学生创新基金项目(SKY0804)
