摘要
目的:研究果胶-酮洛芬(PT-KP)前药对2,4,6-三硝基苯磺酸(TNBS)诱导的大鼠实验性溃疡性结肠炎的治疗作用.方法:采用100mg/kg TNBS诱导大鼠溃疡性结肠炎.将30只溃疡性结肠炎大鼠随机分为PT-KP50,100,200mg/(kg·d)组,模型对照组,正常对照组.除PT-KP50,100,200mg/(kg·d)各组给予不同剂量PT-KP灌胃外,其余各组均给予5mL/L羧甲基纤维素钠溶液灌胃,1次/d,连续6d.实验结束后取大鼠胸腺、脾脏及结肠称质量,计算脏/体系数,并对大鼠结肠进行形态学观察.采用计算机图像处理系统测量溃疡面积并计算溃疡面积百分比.将结肠经40g/L甲醛固定,进行病理组织学检查.实验数据均经统计学处理.结果:PT-KP100,200mg/(kg·d)可使大鼠结肠溃疡面积百分比分别缩小30.43%和5.80%;结肠质量与体质量比减小5.72和3.23.与模型对照组相比较差异均有统计学意义(P<0.05),而对大鼠胸腺及脾脏质量无影响.结论:PT-KP前药对TNBS诱导的大鼠实验性溃疡性结肠炎具有良好的治疗效果.
AIM： To study the curative effect of pectin-keto- profen （PT-KP） prodrug on experimental ulcerative colitis induced by 2, 4, 6-trinitrobenzene sulfonic acid （TNBS） in rats. METHODS： Thirty rats with ulcerative colitis induced with TNBS at the dose of 100 mg/kg were randomized into five groups： PT-KP 50, 100, 200 mg/（kg·d）, model and control. PT-KP 50, 100 and 200 mg/（kg· d） groups were given PT-KP at different doses by intragastric administration, while the other groups were given sodium carboxymethyl cellulose （5 mL/L） once a day for 6 d by intragastric administration. The thymus, the spleen and the colon were taken at the end of the experiment. The weights of thymus, spleen and colon were measured and the ratio of organ weight to body weight was calculated. The morphology of rat colons was observed and the colons were photographed. The ulcerative areas were measured and the percents of ulcerative area were calculated by picture processing system on computer. The colons were fixed in 40 g/L formaldehyde and their histopathologic changes were examined. RESULTS： After the oral administration at the doses of 100 and 200 mg/（kg · d） , the ulcerative area of colon were reduced to 30.43% and 5.80% respectively and the ratios of colon weight to body weight were reduced to 5.72 and 3.23 respectively, significantly lower than those in model group. However, no significant effects were found on the weights of thymus and spleen of the rats at doses of 100 and 200 mg/（ kg· d）.CONCLUSION： PT-KP prodrug has a good therapeutic effect on ulcerative colitis in rats.
出处
《第四军医大学学报》
CAS
北大核心
2009年第13期1231-1234,共4页
Journal of the Fourth Military Medical University
