摘要
分别采用硫酸铵盐析法，丙酮沉淀法，聚乙二醇沉淀法对枯草芽孢杆菌（Ｂａｃｉｌｕｓｓｕｂｔｉｌｉｓ）Ｋ３－１４菌株发酵产生的β－甘露聚糖酶（β－ｍａｎｎａｎａｓｅ）进行提纯．其中硫酸铵盐析法在６０％饱和度时提纯９．１８倍，比活力为１６９．２０Ｕ／ｍｇ；丙酮沉淀法在用量体积分数１．０∶１时提纯１０．４３倍，比活力为１９２．３１Ｕ／ｍｇ；聚乙二醇沉淀法在０．３５ｇ／ｍｌ浓度时提纯１４．６８倍，比活力为２７０．６９Ｕ／ｍｇ．提纯后的β－甘露聚糖酶制品经电泳鉴定呈四条蛋白带．
The βmannanase from Bacillus subtilis K3-14 was purified by (NH4)2SO4 precipitation,Acetone precipitation and PEG precipitation respectively.By (NH4)2SO4 Precipitation (in 60% concentration saturated solution), the βmannanase was purified 9.18 times and its specific activity was 169.20U/mg;by Acetone precipitation (in the volume ratio 1.0∶1 between Acetone and raw enzyme solution), 10.43 times and 192.31[KG*9〗U/mg;by PEG precipitation (in 0.35g/ml concentration solution), 14.68 times and 270.69U/mg.The purified βmannanse product presented four protein bands in PAGE.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1998年第3期200-202,共3页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省政府"18生物资源开发工程办公室"资助
关键词
Β-甘露聚糖酶
枯草芽孢杆菌
纯化
βmannanase,Bacillus subtilis,(NH4)2SO4 precipitation,Acetone precipitation,polyethylene glycol precipitation
