摘要
目的探讨基质金属蛋白酶-9(MMP-9)基因多态性与脑梗死的关系。方法选择山西医科大学第二医院神经内科住院的脑梗死患者57例,健康对照84名。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,根据凝胶电泳所见的条带区分各研究对象的基因型,并统计各基因型频率。与健康对照组上述指标进行比较。结果脑梗死组MMP-9 C-1562TT/T基因型与健康对照组比较差异有统计学意义(P<0.05)。结论MMP-9C-1562TT/T基因型与脑梗死明显相关,该多态性可能是导致脑梗死后MMP-9表达增加的原因。
Objective To investigate the relevance between polymorphism of matrix metalloproteinase-9 （MMP-9） and cerebral infarction. Methods Functional single nucleotide polymorphisms （SNPs） of MMP-9 C-1562T were ex- amined by polymerase chain reaction-restriction fragment length polymorphism to identify their merits as genetic mark- ers for cerebral infarction （CI） in 57 patients and in 84 control subjects participated in this study. The genotypes as shown by electrophoresis and frequencies of alleles were compared between the patients and controls. Results Three genotypes of MMP-9 （C/C, C/T and C/T） were detected in both groups. The frequency of T/T genotype in CI was sig- nificantly higher than those in the control group （P〈0.05）. Conclusion The MMP-9 C-1562T T/T genotype was sig- nificandy associated with CI, which may be underlying the increased MMP-9 expression after CI.
出处
《中国药物与临床》
CAS
2009年第7期575-577,共3页
Chinese Remedies & Clinics
基金
山西省自然科学基金(20051100)
山西省归国留学人员基金(2005069)
关键词
脑梗塞
明胶酶β
基因多态性
Brain infarction
Gelatinaes β
Gene polymorphism
