摘要
目的 建立外周血基因组DNA 的快速提取方法。方法 用碘化钾直接从外周血中提取基因组DNA。结果 用该方法提取的DNA 为大分子量DNA;A260/A280为1.85,A260/A230为2.2;提取效率大于90% ;DNA 体外扩增结果良好。结论 该方法简便、快速、经济,可用于大量临床标本的研究。
Objective To establish the method of genomic DNA extraction.Methods Genomic DNA was directly extracted from peripheral blood with the use of KI.Results The genomic DNA extracted with KI was of large molecular weight.A 260 /A 280 and A 260 /A 230 were 1.85 and 2.2 respectively. The extraction efficiency was greater than 90%.The result of PCR for the DNA was good.Conclusion The method is simple,quick,economical and could be used for studying numbers of clinical samples.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1999年第6期395-396,共2页
Chinese Journal of Medical Genetics
关键词
基因组DNA
碘化钾
提取
外周血
Genomic DNA Potassium iodide Extraction Peripheral blood
