摘要
目的研究苦豆子生物碱衍生物施普睿达对荷H22肝癌小鼠基因表达谱的影响,探讨施普睿达抗肝癌的分子机制。方法应用包含4096个小鼠基因的cDNA芯片检测施普睿达对荷H22肝癌小鼠基因表达谱的影响,并对差异基因进行GO聚类和统计分析。结果施普睿达治疗前后共有358个基因出现差异表达,83个基因的表达差异达到3倍以上,表达差异在2倍以上的基因共有275个。经GO分析,差异表达基因包括许多与细胞凋亡、细胞周期、代谢、免疫应答和DNA修复等相关的基因。结论施普睿达主要是通过调控肿瘤细胞周期进程、影响代谢和免疫应答、调节肿瘤细胞凋亡相关基因的表达等多种途径抑制肿瘤的增殖和转移。
Objective To investigate the effects of SPRIDA, an alkaloid derivative from Sophora alopecuoride, on gene expression profile in mice bearing H22 liver cancer and explore the potential mechanism for its action against liver cancers. Methods The mouse cDNA microarray containing 4096 genes were used to detect the gene expression profile induced by SPRIDA in mice bearing H22 liver cancer, while the gene ontology clustering and statistic analysis of differential expression genes were performed. Results Before and after treatment with SPRIDA, the mice bearing H22 liver cancer showed 358 genes with differential expression. Eighty-three genes showed more than three times, and 275 showed two times as much in expression difference. Gene ontology analysis showed that most of differential expres- sions involved genes related with cell cycling, apoptosis, metabolism, immune response and DNA repair. Conclusion SPRIDA may inhibit tumor growth and metastasis through modulation of gene expressions related with cell cycling, metabolism, immune response and apoptosis.
出处
《中国药物与临床》
CAS
2009年第7期563-565,共3页
Chinese Remedies & Clinics
基金
科技部科研院所技术研究开发专项(2004EG136193)
天津市科技支撑计划重点项目(07ZCKFSH00200)
关键词
肝肿瘤
基因表达谱
肿瘤负荷
Liver neoplasms
Gene expression profiling
Tumor burden
