摘要
目的:探索慢性宫内缺氧时胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)在新生兔脑组织和血清中的表达规律。方法:取孕中晚期新西兰兔24只,随机分为对照组、缺氧组以及中药干预缺氧组,对比3组新生兔的脑组织和血清的IGF-1表达与变化。结果:IGF-1在脑组织的表达经χ2检验,各组间差异均有统计学意义(P<0.01),缺氧组明显高于中药干预组,且中药干预组高于对照组。ELISA法定量测定血清IGF-1含量,缺氧组的血清IGF-1的含量低于对照(P<0.01),中药干预组的血清IGF-1的含量低于对照组(P<0.05)。结论:缺氧缺血性脑病(Hypoxic ischemic encephalopathy,HIE)新生兔的体内IGF-l进行了重新分布。脑组织内为保护神经细胞发生应激反应,调集外周血中的IGF-1进入中枢神经系统,参与内源性保护机制。
Objective： Searching after the regularity of the expressions the insulin-like growth factors （IGF-1） inside the neonatal rabbits with chronic intrauterine anoxemia give to brain Tissues and serums. Methods： Choosing 24 Zelanian rabbits that have been in afternoon pregnancy,we divide them into three stochastic eontratest group by chance： the control group,the anoxic group and the group interfered with Chinese traditional medicine. Then we contrast the expressions and changes of these groups with IGF-1 in brain tissues and in serums. Results： Contrasting IGF-1 in brain tissues,checked up withΧ^2. In three different groups, we have found that the differences of their expressions have some statistical significance （P 〈0.01 ）;IGF-1 in brain tissues of the anoxic group is higher than the group interfered with Chinese traditional medicine and IGF-1 in brain tissues of the group interfered with Chinese traditional medicine is higher than the control group.Determined the contents of IGF-1 in serums on rabbits by ELISA,the contents of IGF-1 in serums of the anoxic group is extremely remarkably lower than the control group （P 〈0.01 ）, while the contents of IGF-1 in serums of the group interfered with Chinese traditional medicine is extremely remarkably lower than the anoxic group. Conclusion：In this article,there occurs the mentions： IGF-1 has been redistributed in the bodies of HIE neonatal rabbits;Irritable reactions inside brain tissues have been brought into existance in order to protect nerve cells, then IGF-1 from the peri-islet extro-blood all assembled into the central nervous system, then participate in the intrinsic protection mechanism.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第7期895-898,共4页
Journal of Chongqing Medical University
基金
福建省教育厅基金项目(JB07203)
泉州市科技局基金项目(2007Z50)
关键词
胰岛素样生长因子-1
HIE
新生兔
脑组织
血清
Insulin-like growth factor- 1 （IGF- 1 ）
Hypoxic ischemic encephalopathy（ HIE ）
Neonatal rabbits
Brain tissues
Serums
