摘要
目的:建立以高效液相色谱法同时测定黄连解毒汤中黄芩苷、盐酸小檗碱、栀子苷含量的方法。方法:色谱柱为岛津Sim-packVP-ODSC18(250mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸二氢钾+0.05%三乙醇胺溶液(磷酸调pH3.0,梯度洗脱),检测波长为254nm。结果:栀子苷、盐酸小檗碱、黄芩苷的检测浓度分别在0.4～2.0mg.mL-1(r=0.9981)、0.020～0.40mg.mL-1(r=0.9920)、0.020～0.40mg.mL-1(r=0.9963)范围内与各自峰面积积分值呈良好的线性关系;三者加样回收率分别为99.8%、97.5%、98.1%,RSD分别为1.8%、2.5%、1.5%(n均=6)。结论:本方法专属性强、灵敏度高、操作简便,适用于黄连解毒汤类制剂的质量控制。
OBJECTIVE： To establish an HPLC method for the determination of the contents of baicalin, berberine hydrochloride and jasminoidin in Coptidis decoction for detoxification. METHODS： The samples were separated on Shimazu Sim -pack VP -ODS C18（250 mm × 4.6 mm, 5μm） with mobile phase consisted of acetonitrile - （0.05% potassium dihydrogen phosphate ＋ 0.05% trolamine solution, with pH adjusted to 3.0 by phosphoric acid） in gradient elution under a detection wavelength of 254 nm.RESULTS： The linear ranges of jasminoidin, berberine hydrochloride and baicalin were 0.4-2.0 mg · mL^-1（r = 0. 998 1）, 0.020-0.40 mg·mL 1（ r = 0. 992 0） and 0.020- 0.40 mg · mL^-1（r = 0. 996 3）, respectively, and their average recoveries were 99.8%, 97.5 % and 98.1%, respectively, with RSD at 1.8 %, 2.5 % and 1.5 %, respectively（ n = 6 for all the three）.CONCLUSION： The established method is specific, sensitive, simple and applicable for the quality control of Coptidis decoction for detoxification.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第18期1410-1411,共2页
China Pharmacy
关键词
高效液相色谱法
黄芩苷
盐酸小檗碱
栀子苷
含量测定
黄连解毒汤
HPLC
Baicalin
Berberine hydrochloride
Jasminoidin
Content determination
Coptidis decoction for detoxification
