摘要
[目的]建立采用高效毛细管电泳色谱法测定肉苁蓉中有效成分红景天苷含量的方法。[方法]采用毛细管区带电泳（CZE）：运行缓冲液为30mmol／L硼酸-硼砂缓冲液（pH=9．00）；工作电压8kV；柱温25℃；检测波长278nm。[结果]红景天苷含量在0．007—0．140mg／ml的浓度范围内线性关系良好（r=0．9998），加样回收率为1130．1％，RSD为1．4％。[结论]高效毛细管电泳法可用于测定肉苁蓉中红景天苷的含量，该方法简便、快速、准确、重复性好。
[ Objective ] The research aimed to establish a HPCE method for determining salidroside in Herba Cistanches. [ Method ] Using the CZE, salidroside content was determined in Herba Cistanches and the experimental conditions were as foUows： 30 mmol/L boron granule buffer （pH =9.0）, working voltage of 8 kV, temperature of 25 ℃ and detective wavelength of 278 nm. [ Resuh] The linear range of salidroside was 0.007 -0. 140 rag/m｜ （r =0.999 8） and the average recovery was 100.1% （RSD = 1.4% ） . [Conclusion] The established method is simple, quick and accurate and suitable for determining salidroside in Herba Cistanches.
出处
《安徽农业科学》
CAS
北大核心
2009年第19期8979-8980,共2页
Journal of Anhui Agricultural Sciences
