摘要
[目的]探讨Tricine-SDS-PAGE技术应用于饲料蛋白质的分析与品质鉴定的可行性。[方法]采用Tricine—SDS—PAGE对菜籽饼粕、棉籽饼粕、菜籽浸出粕、热带假丝酵母固态发酵棉籽粕以及黑曲霉固态发酵棉籽粕分别进行凝胶成像分析，鉴定5种不同品质饲料的蛋白质组成。[结果]采用4．0％C、16．5％T的分离胶，可以取得较好的分离效果。菜籽蛋白和棉籽蛋白样本的凝胶成像结果表明，菜籽饼粕中的蛋白质主要集中在泳道中部，为14．4～60．0kD，而棉籽饼粕中的蛋白则在泳道顶端（约90kD）含量较高，2种饲料蛋白得到了有效区分。同一种样本相同处理方法间的凝胶成像结果表明，用黑曲霉对棉籽饼粕进行固态发酵效果优于热带假丝酵母。[结论]5种饲料之间蛋白成像差异显著、直观，Tricine-SDS—PAGE可以作为饲料蛋白品质鉴定的一种有效方法。
[ Objective] The feasibility of the identification of protein and the analysis of the quality of different feedstuff with Trieine-SDS- PAGE system was discussed. [ Method ] The protein composition of five feedstuffs, rapeseed meal, cottonseed meal, extract from rapeseed meal, cottonseed meal fermented by Monilia tropicalis and cottonseed meal fermented by black mold, was identified with Tricine-SDS-PAGE system. [ Results] The better isolation efficiency could be produced with 4.0% C and 16.5% T. The protein band of rapeseed meal was showed in the central part（ 14.4 - 60.0 kD） and cottonseed meal, end part（ about 90 kD） ,which contain was higher among all of experimental materi- als and the protein in the method was easy to be effectively separated. The result indicated that the effect of the cottonseed meal treated fer- mented by black mo/d was better that fermented by Monilia tropicalis. [ Conclusion] There was significant different in protein image among five feedstuffs and the method of Tricine-SDS-PAGE ought to be the effective method in identification of the protein in feedstuff.
出处
《安徽农业科学》
CAS
北大核心
2009年第19期8909-8910,共2页
Journal of Anhui Agricultural Sciences
基金
国家"十一五"科技支撑计划课题(2006BAD12B04)"农副产品饲料转化和高效利用技术研究"
