摘要
对一些小分子多肽进行电泳分析时，在固定、染色、脱色过程中极易扩散丢失；即使用常规尿素－ＳＤＳ－ＰＡＧＥ系统测定寡肽分子量，银染色后小于８ｋＤａ的标准品也无着色带显示。我们实践摸索出提高分离胶的浓度和交联度，制成２０％Ｔ，６％Ｃ和１５％Ｔ，３％Ｃ的梯度胶，胶中不加尿素，且不需银染均可见小分子多肽着色带的方法。
Abstract SDS PAGE is particularly molecular useful for the analysis of multi component peptides,but the low weights peptides is easy to diffuse during fixing,staining,deslaining,By using normal urea SDS PAGE system with silver slaining we still have not see any staining band of molecular weights below 8kD,Here we describe one method which increases the staining band sensitivity by rising the concentration and the degree of crosslinking of the separation gels.
出处
《生物工程进展》
CSCD
1998年第6期49-51,共3页
Progress in Biotechnology
