摘要
[目的]研究Na^＋／H^＋逆向转运蛋白AtNHXl基因对杨树的转化。[方法]以杨树南林895杨的叶片作为外植体，采用根癌农杆菌介导法，构建cre／lox植物表达载体，并对3株转基因植株进行PCR分子检测。[结果]结果表明，较适合的转化条件为：外植体在分化培养基上经过2d预培养后于0D600nm值为0．3～0．4的菌液中浸泡7min，卡那抗性绿苗率可达43．9％；PCR分子检测表明，目的基因已经整合到杨树基因组中。[结论]该系统可以应用于杨树的基因工程研究。
[ Objective ] The poplar transferred with Na^＋/H^＋ anti-porter AtNHX1 gene was studied. [ Method ] The leaf of the poplar variety - - Nanlin 895 being taken as explants, the expression vector cre/lox was set up with Agrobacterium-mediated method, and 3 transgenic plants were detected at molecular level with PCR. [ Results] The results showed that the condition of suitable conversion was as follows that after tile explants were pre-cultured in the differentiation medium for 2 days, its soaking time in the bacterium with OD600nm value of O. 3 - 0.4 was 7 minutes. And then, the ratio of kanamycin-resistant green plant could be 43.9%. PCR molecular detection showed that the target gene had been integrated into poplar genome. [ Conclusion ] The system could be applied in gene transformation of poplar
出处
《安徽农业科学》
CAS
北大核心
2009年第19期8874-8875,8921,共3页
Journal of Anhui Agricultural Sciences
基金
南京晓庄学院重点项目(2007NXY01)
生态学校级重点学科项目(2005-2008)
江苏省高校自然科学基金项目(08KDJ180011)
江苏省高等学校大学生实践创新训练计划项目(2007-2009)
