摘要
根据柽柳cDNA文库中获得的eIF-5A基因片段,用RACE技术克隆出其全长cDNA序列。cDNA长度为799bp,编码159个氨基酸。将该cDNA序列克隆到原核表达载体pET28a中,获得重组质粒pET28a-eIF5A。不同浓度NaCl胁迫下大肠杆菌(Escherichiacoli)BL21(pET28a-eIF5A)比E.coliBL21(pET28a)有明显的抗盐性,前者菌株存活率在1.0mol·L-1NaCl盐胁迫下是后者的9.3倍,据此认为E.coliBL21(pET28a-eIF5A)的耐盐性可能与eIF-5A基因的表达相关。该基因的GenBank登录号为AY587771(基因)、AAT01416(蛋白)。
Based on the partial sequence of eIF-5A gene acquired from Tamarix androssowii cDNA library, the full length cDNA sequence of eIF-5A was isolated using RACE technology. The cDNA sequence was 799 bp in length and encoded a deduced amino acid sequence of 159 residues. The gene was cloned into pET28a-vector and the recombination vector was named pET28a-eIF5A. The salt-tolerance experiment showed Escherichia coli BL21 （pET28a-eIF5A） had more salt-resistance ability than E. coli BL21 （pET28a） at different NaC1 concentrations and the former was 9.3 times in survival rate to the later under the stress of 1.0 mol·L^-1 NaCl. This indicated that the high ability of salt-tolerance of E. coli BL21 （pET28a-eIF5A） might be related to the expression of eIF-5A gene. The elF-5A gene was accepted by GenBank, the accession numbers are AY587771 （gene） and AAT01416 （protein）.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2005年第4期433-438,共6页
Plant Physiology Communications
基金
国家重点基础研究发展规划"(973")项目(G1999016000)
国家转基因植物研究与产业化专项(JY03A1802)。
