摘要
利用农杆菌介导的转化系统转化中花 11成熟胚愈伤组织 ,获得 14 89个独立转化的T DNA插入再生株系。PCR和Southern杂交的结果表明 ,6 9 8%转化株系被整合了T DNA ,通过Tail PCR也从转化植株中扩增出T DNA侧翼序列。同时对 10 6 6个T1转化株系的抽穗期、株高、单株穗数的调查结果表明 ,不同株系中分离出了突变植株。
By using the matured embryos of Japonica rice variety Zhonghua No.11 as explants,rice transformation was performed by Agrobacterium-mediated co-cultivation method,resulting in 1 489 independent transgenic rice plants that carry a T-DNA insertion.Genomic DNA gel-blot and PCR analyses showed that 69.8% of the total lines contain the inserted T-DNA.The flanking sequence of T-DNA in transgenic rice plants was analyzed using Tail-PCR.In addition,we have evaluated 1 066 T_1 transgenic lines on heading days,plant height and panicles per hill,and found different types of mutants from a number of lines.
基金
国家转基因植物专项 (编号 :ZJY A 0 8)
国家 863高技术项目 (编号 :2 0 0 1AA2 2 5 0 12 )资助~~
关键词
T-DNA插入突变
整合
抽穗期
株高
单株穗数
T-DNA insertional mutagenesis
integration
heading days
plant height
panicles per hill
