摘要
目的探讨MMP-2和VEGF在卵巢癌组织中的表达及其与卵巢癌发生发展的关系,本研究拟从基因和蛋白水平研究二者在卵巢癌组织中的表达情况。方法运用逆转录聚合酶链反应(RT-PCR)和免疫组织化学染色法检测MMP-2和VEGF在正常卵巢组织、卵巢癌组织中的表达情况。结果卵巢癌组织中MMP-2和VEGFmRNA表达水平及蛋白阳性率均高于正常卵巢组织(P<0.05),MMP-2、VEGFmRNA表达水平及蛋白阳性表达率与卵巢癌的浸润程度、临床分期和淋巴结转移有关(P<0.05)。结论正常卵巢组织和卵巢癌组织中MMP-2、VEGFmRNA和蛋白含量的变化是一致的;MMP-2、VEGF可能参与了卵巢癌的发生、侵袭和转移;MMP-2、VEGF定量检测可能成为卵巢肿瘤恶化的分子指标及卵巢癌治疗的分子靶标之一。
Objective To study and explore the expression of MMP-2 and VEGF in ovarian carcinomas, and investigate their correlations to oncogenesis and progression of ovarian cancer.Methods The mRNA and protein levels of MMP-2 and VEGF in 42 sampies of ovarian cancer and 18 samples of normal ovarian tissue were detected with reverse transcription-polymerase chain reaction （RT- PCR） and immunohistochemistry. Results The mRNA levels and positive rate of MMP-2 and VEGF proteins were significantly higher in ovarian cancer than that in normal ovarian tissue（ P 〈 0.05）. There was significant relationship between the mRNA levels and positive rates of MMP-2 and VEGF proteins and the depth of invasion, TNM stage, lymph node metastasis. Conclusion The changes in mRNA levels of MMP-2 and VEGF in ovarian cancer are accordant to the changes in positive rates of the proteins, MMP-2 and VEGF were important for the tumor invasion and metastasis. Expressions of MMP-2 and VEGF in ovarian carcinomas appear to be well-characterized indicators of tumor prognosis and metastasis.
出处
《中国实验诊断学》
北大核心
2009年第6期726-729,共4页
Chinese Journal of Laboratory Diagnosis
基金
佳木斯大学科研立项资助项目(S38)
关键词
卵巢癌
MMP-2
VEGF
逆转录聚合酶链反应
免疫组织化学
Ovarian cancer
Matrix metalloproteinase-2
Vascular endothelial growth factor
RT-PCR
immunohistochemistry
