摘要
目的：了解慢性丙型肝炎患者的血浆及其外周血单个核细胞（ｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕｃｌｅａｒｃｅｌｓ，ＰＢＭＣ）中丙型肝炎病毒（ｈｅｐａｔｉｔｉｓＣｖｉｒｕｓ，ＨＣＶ）的存在及存在形式。方法：反转录多聚酶链反应（ｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＲＴＰＣＲ）技术检测１２４例慢性丙型肝炎患者的血浆及ＰＢＭＣ中的ＨＣＶ。结果：在７０例抗ＨＣＶ及血浆ＨＣＶＲＮＡ阳性的患者中有２１例（３０％）的患者其ＰＢＭＣ内存在ＨＣＶＲＮＡ正链，仅１例存在ＨＣＶＲＮＡ负链。在５４例抗ＨＣＶ阳性、血浆ＨＣＶＲＮＡ阴性的患者中，仅有２例（３．７％）检出ＨＣＶＲＮＡ正链，未检出ＨＣＶＲＮＡ负链，这２例患者均为干扰素治疗３～６个月的病人。结论：ＰＢＭＣ可作为肝外病毒复制场所。对慢性ＨＣＶ感染者来说，３～６个月的干扰素治疗时间可能是不够的，ＨＣＶ在ＰＢＭＣ内的存在可能是干扰素治疗后复发的原因之一。
Objective: To study HCV RNA in plasma and its peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis C. Methods: Detection of HCV RNA in plasma and PBMC of patients with chronic hepatitis C by RT-PCR. Results: In 70 patients whose antiHCV and HCV RNA were positive in their plasma with chronic hepatitis C, HCV RNA positive strands in 21 patients(30%) and HCV RNA negative strand in 1 patient were detected by RT-PCR. The HCV RNA positive strands of 2/54(3.7%) patients whose antiHCV was positive and HCV RNA was negative in their plasma with interferon treatment for 3 or 6 months were detected,and no HCV RNA negative strands in their PBMCs were found by RT-PCR. Conclusion:These results suggest that HCV can infect PBMCs and replicate in it, and demonstrate that HCV infection of PBMCs exists in patients with HCV RNA seropositive more easily than those with HCV RNA negative in their sera. Interferon treatment for three or six months was inadequate in some patients, because HCV RNA in their PBMCs may be detected.
出处
《北京医科大学学报》
CSCD
1998年第3期221-223,共3页
Journal of Peking University(Health Sciences)
关键词
丙型肝炎
丙型肝炎病毒
单核细胞
血液
Polymerase chain reaction/methods Hepatitis C viruses/isol Monocytes/blood Interferons/ther use
