摘要
采用考马斯亮蓝法,对不同果汁中的蛋白质含量进行了测定。结果表明:蛋白质浓度在10-80μg/mL内与吸光度有良好的线性关系(R2=0.9964);样品平均回收率(n=3)分别为99.2%、98.8%、101.8%,RSD分别为0.64%、0.81%和0.58%(n=3)。该方法具有快速、灵敏度高、重现性好等特点。
The content of proteins in different juices was determined by the dying method with coomassie brilliant blue. The result showed that there was a good linearity relation between the absorbency and the content of standard proteins in the range of 10--80μg/mL. The average recovery rate were 99.2%, 98.2%, 101.8%, respectively, RSDs were 0.64%, 0.81% and 0.58% respectively. A rapid, sensitive, and accurate method for the determination of protein content in juice was found.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2009年第5期160-162,共3页
Food and Fermentation Industries
基金
陕西省农业科技攻关计划(2006K02-G4)
关键词
果汁
蛋白质
考马斯亮蓝
含量
测定
juice, protein, coomassie brilliant blue, content, determination
