摘要
目的:研究轻肝颗粒防治脂肪肝的作用。方法:小鼠腹腔注射四环素100mg/kg.d×7d加灌胃高脂乳剂(10%猪油加5%胆固醇)10ml/kg.d×14d复制成非酒精性脂肪肝模型,轻肝颗粒分别灌胃给予低、中、高(4.5g/kg、9.0g/kg、18.0g/kg)三个剂量,阳性组灌胃给予辛伐他汀(0.01g/kg),测定血清总胆固醇(TC)、甘油三酯(TG)、高密度胆固醇(HDL-C)、低密度胆固醇(LDL-C)、总蛋白(TP)、白蛋白(Alb)含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)活性;制备肝匀浆并测定肝脏TG、TC水平;取肝脏称湿重计算其脏器指数并作肝组织病理组织学检查。结果:与正常对照组相比较,造模组动物血清HDL-C、LDL-C、TG水平及AST活性显著降低,肝脂质TG、TC含量及肝脏病理评分显著增加。轻肝颗粒可增加该脂肪肝模型小鼠血清LDL-C水平和VLDL-C水平,可显著降低血清ALP、ALT活性,显著降低脂肪肝小鼠肝脏TG、TC水平及其总TG的含量,减少肝脏脂质堆积,减少其病理积分,与模型组比较有统计学差异;但轻肝颗粒对该脂肪肝模型小鼠血清TG、TP水平及A/G比值及AST活性均无明显影响。结论:轻肝颗粒防治脂肪肝作用可能与其防止肝脏脂肪堆积、调节肝脏脂质代谢等作用有关。
Objective： To study the effects of Qinggan Granule on non-alcoholic fatty liver in mice. Methods： Mice non-alcoholic fatty liver were induced by ip tetracycline hydrochloride 100mg/kg once a day for 7 days added ig high fat diet（ 10% lard and 5% cholesterol） 10ml/kg once a day for 14 days. Meanwhile mice were separately ig Qinggan Granule 4.5, 9.0 or 18.0g/kg. The control group and the model group animal were given the same volume of 0.5% CMC-Na. The levels ofTC, TG, HDL-C, LDL-C, TP and Alb or the activity of ALT, AST and ALP in serum were detected. The levels of TG and TC in livers also were detected. Calculated the hepatic indexes and checked the morphological changes of the liver tissues under microscope. Results： These results showed that tetracycline hydrochloride and high fat diet significantly decreased the level of HDL-C, LDL-C and TG and the activity of AST in serum, increased the content of TG, TC in liver, and index of liver in mice. Qinggan Granule significantly increased the levels of LDL-C and VLDL-C, reduced the activities of ALP and ALT in serum and the content of TG , TC in liver in NAFL mice. Conclusions： It suggested that the effects of Qinggan Granule on NAFL may be relating to regulation of the metabolism of lipids and reduce lipids accumulated in liver.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第2期94-97,共4页
Pharmacology and Clinics of Chinese Materia Medica
