摘要
目的探讨胃癌组织中微血管密度(microvessel density,MVD)与CD105、CD31的表达及生物学行为的关系。方法取61例术前未作放、化疗及免疫治疗胃癌病人的外科手术切除标本,采用免疫组织化学技术S-P法检测CD105、CD31的表达,并定量计数胃癌组织微血管。结果胃癌组织中CD105及CD31标记的MVD在与淋巴结转移、肿瘤浸润深度均有显著性差异(P<0.05);Kaplan-Meier法分析显示微血管计数CD105<14个,CD31<34个(200×)的胃癌病人预后较好,而微血管计数CD105≥14个,CD31≥34个(200×)预后较差。结论胃癌MVD与其生物学行为有关,提示肿瘤血管生成分析和微血管密度计数是评价胃癌预后的一项重要参考指标。
Objective To investigate the microvessel density （MVD） and the expressions of CD105 and CD31 in the tissue of gastric carcinoma and their relationship with tumor invasion and metastasis, and to evaluate the relationship of MVD value with the prognosis of patients with gastric carcinoma. Methods After operation, section preparation of 61 patients with gastric carcinoma without radiotherapy, chemotherapy or immunotherapy before operation were examined by using S-P immunohistochemical method to detect the expressions of CD105 and CD31 , and the microvessel in the tumor tissue was counted quantitatively. Results Under the field of vision of 200 times, the value of MVD marked with CD105 and CD31 in the gastric carcinoma had significance difference in both the depth of carcinoma invasion and metastasis of lymphatic nodes （P 〈 0.05）. Kaplan-Meier analysis showed that patients with CD105 〈 14 and CD31 〈 34 （200×） had better prognosis and that patients with CD105≥14 and CD31 ≥34 （200×） did not. Conclusion The value of MVD in the gastric cancer is correlated with the biological behavior of the carcinoma, which suggests that the analysis of the genesis of tumor vessel and the count of MVD are an important index in evaluation the prognosis of patients with gastric cancer.
出处
《胃肠病学和肝病学杂志》
CAS
2009年第6期524-526,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
胃癌
CD105
CD31
微血管密度
免疫组化
Gastric cancer
CD105
CD31
Microvessel density （MVD）
Immunohistochemistry
