摘要
以可生物降解高分子聚酯－醚嵌段共聚物聚－Ｄ，Ｌ－乳酸－聚乙二醇（ＰＬＡ－ＰＥＧ）为基质材料，采用水／油／水复合乳液溶剂蒸发技术制备载蛋白的微球（ＭＳ）。蛋白的加入改变了共聚物微球的结晶性能，差示扫描量热（ＤＳＣ）分析反映出结晶度下降及多重结晶状态。采用溶剂萃取法及紫外吸收对微球中蛋白含量作定量分析，表明蛋白携载量与蛋白性质及蛋白加入量有关。适当加入表面活性剂可改善微球对蛋白的包接性能。
Biodegradable polylactidebpolyethylene glycol(PLAPEG) microspheres with micron size carrying human serum albumin(HSA) or outer membrane protein(OMP) were prepared out by waterinoilinwater composite emulsion solvent evaporation technique. The data of DSC showed a reduction of the heat of fusion ΔHp=2.161 J/g of protein carrying PLAPEG microspheres from ΔH0=25.683 J/g of non proteincarrying PLA PEG microspheres which indicated that the encapsulation of protein changed the crystallinity of the PLAPEG microspheres. The increase of the peaks of endothermic fusion of crystalline of the DSC curve of the proteincarrying PLAPEG microspheres identified the effective encapsulation of protein in microspheres. The quantitative analysis of protein carried out by sampling of abstraction and ultraviolet absorbance at 283 nm showed that the content of protein in PLAPEG microspheres was related to the nature of protein and the amount of protein in feed. The content of protein in PLAPEG microspheres can reach 10%. The adding of surfactant in emulsion improved the encapsulation of protein in PLAPEG microspheres.
出处
《高分子材料科学与工程》
EI
CAS
CSCD
北大核心
1998年第4期93-96,共4页
Polymer Materials Science & Engineering
基金
国家自然科学基金
关键词
蛋白包裹
生物降解
高分子微球
疫苗
polylactidebpolyethylene glycol, microspheres, HSA, OMP, encapsulation
