摘要
Ｍｏｄｕｌａｔｉｎｇｅｆｅｃｔｏｆｍｉｔｏｍｙｃｉｎｏｒｃｉｓｐｌａｔｉｎｏｎｌｙｍｐｈｏｋｉｎｅａｃｔｉｖａｔｅｄｋｉｌｅｒｃｅｌｐｒｏｌｉｆｅｒａｔｉｏｎａｎｄａｎｔｉｔｕｍｏｒａｃｔｉｖｉｔｙｔｏｂｌａｄｄｅｒｃａｎｃｅｒｃｅｌｌｉｎｅｓｉｎ...
AIM: To study the effect of mitomycin (Mit) or cisplatin (Cis) on the proliferation of lymphokine activated killer (LAK) cells in patients with transitional cell cancer of bladder and their cytolysis to bladder tumor cells. METHODS: LAK cell proliferation was assayed in the presence of Mit or Cis by cell counting. Bladder cancer cell lines BIU 87 and EJ were cultured as target cells and cytotoxicity of LAK cells was determined by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay. RESULTS: The proliferation of LAK cells induced by recombinant interleukin 2 (IL 2) was inhibited by Cis in a concentration dependent manner and was decreased to 55 3 % at 100 mg·L -1 compared with control at 96 h . The enhanced growth of the LAK cells was observed with Mit 5-10 mg·L -1 from 48 to 96 h . Cis 10 mg·L -1 increased the cytotoxicity against BIU 87 and EJ cells. CONCLUSION: Immunomodulatory effect of chemotherapeutic agents on LAK cell proliferation induced by IL 2 in patients with bladder cancer mainly depends on the drug itself.
出处
《中国药理学报》
CSCD
1998年第4期369-372,共4页
Acta Pharmacologica Sinica
关键词
丝裂霉素C
顺铂
淋巴因子
杀伤细胞
膀胱癌
mitomycin C
cisplatin
lymphokine activated killer cells
bladder neoplasms
cell division
interleukin 2
immunologic cytotoxicity
cultured tumor cells
Keywords mitomycin C
cisplatin
lymphokine activated killer cells
bladder neoplasms
cell
