摘要
A型流感病毒能诱导淋巴细胞、单核巨噬细胞的凋亡,为进一步探讨淋巴细胞和单核巨噬细胞在凋亡中可能存在的相互作用,用H1N1亚型流感病毒诱导人外周血淋巴细胞和单核巨噬细胞的凋亡.结果显示,前48h,H1N1流感病毒能诱导淋巴细胞和单核巨噬细胞的凋亡,但在培养48h后,流感病毒对单核巨噬细胞表现为凋亡抑制作用,同时流感病毒对淋巴细胞吸附不同时间后,荧光染色和流式细胞术检测凋亡未见明显差异,说明细胞凋亡与病毒吸附时间长短并无相关性.检测p53抑制剂Pifithrin-α(PFT-α)加入前后淋巴细胞和单核巨噬细胞的凋亡情况,结果显示,淋巴细胞和单核巨噬细胞的凋亡均被抑制,提示通过p53诱导的凋亡可能是流感病毒诱导细胞凋亡的一条重要途径.
Peripheral blood mononuclear cell （PBMC） was induced apoptosis when infected by influenza A virus. To observe the interaction between lymphocyte and macrophage, the lymphocyte and macrophage were induced by H1N1 subtype influenza virus. The result showed that apoptosis of PBMC including lymphocyte and macrophage was promoted by virus infection in the first 48 h, but the apoptosis of macrophage was inhibited after 48 h cultivation. According to fluorescent staining and flow cytometry there was no difference in apoptosis of lymphocyte after virus adsorption at different time. The result showed that there was no relationship between apoptosis and adsorption time. The apoptosis of lymphocyte and macrophage was detected when PFT-α was added, the result show that the apoptosis of lymphocyte and macrophage was inhibited. It hinted that p53 may be impact the apoptosis of lymphocyte and mononuclear macrophage that treated with influenza virus.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2009年第6期770-775,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金(30771988,30571674)
广东省自然科学基金(05008347,04020239)
广东高校优秀青年创新人材培育项目(LYM08056)资助~~
关键词
外周血单个核细胞
A型流感病毒
凋亡
P53
peripheral blood mononuclear cell, influenza A virus, apoptosis, p53
