摘要
常用纤维蛋白原的吸附特性来比较和研究材料的血液相容性。将自行合成的正电磷脂ＤＰＰＥＬ膜铺展在金膜表面，测量了膜表面的接触角及对纤维蛋白原的动态吸附特性，并与裸金膜、中性磷脂ＤＳＰＣ膜进行了比较。结果表明：ＤＳＰＣ膜、ＤＰＰＥＬ膜和裸金膜的接触角分别为３８°０５′，５７°２０′和７８°２４′。在相同的纤维蛋白原溶液浓度条件下，裸金膜表面吸附纤维蛋白原的速率最快，饱和吸附量最大（３．５０ｎｇ／ｍｍ２）；磷脂ＤＳＰＣ膜表面吸附纤维蛋白原的速度最低，饱和吸附量也最小（１．００ｎｇ／ｍｍ２）；磷脂ＤＰＰＥＬ膜吸附速率也较快，饱和吸附量为１．７５ｎｇ／ｍｍ２。表明纤维蛋白原在界面上的吸附特性与材料表面疏水性质和带电特性相关。ＰＣ头良好的亲水性和电中性，是其能改善生物医用材料血液相容性的重要原因。
The adsorption of fibrinogen at different phospholipid surfaces was studied. For comparing the effect of different charges, a cationic phospholipid dipalmitoylLαphosphatidylethanolamine covalently coupled to Llysine (DPPEL) was synthesized. A neutral phosphatidylcholine (PC) layer and a cationic DPPEL layer were formed on gold surface by spin coating. The two phospholipid membrane and gold membrane surface appeared different contact angles, indicating their different hydrophilicity. The adsorption process of fibrinogen from buffer onto different surface was investigated using simultaneous surface plasmon resonance (SPR) biosensor made in this laboratory. It was found that phosphatidylcholine surface without net charge and with a high hydrophilicity, gives a lower adsorption of fibrinogen than the cationic DPPEL surface. The gold surface with a hydrophobicity, gives a highest fibrinogen adsorption. The result shows that the headgroup of phospholipid has a major effect on the fibrinogen adsorption. Electrostatic and hydrophobic interaction between the protein and the surface are two major factors involved in the adsorption mechanism.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1998年第6期59-62,共4页
Journal of Tsinghua University(Science and Technology)
基金
国家自然科学基金
关键词
磷脂
纤维蛋白原
表面吸附
生物传感器
血液
phospholipid
fibrinogen
surface adsorption
surface plasmon resonance (SPR) biosensors
