摘要
本文以盐生植物盐芥和甜土植物拟南芥为实验对象,利用生物信息学手段分析两者ABA受体的进化关系和保守结构域,比较和研究了盐芥和拟南芥在400 mmol·L-1甘露醇、200 mmol·L-1 Na Cl和50μmol·L-1 ABA处理下的根生长,相对含水量和渗透压等生理指标的差异及ABA受体基因在胁迫条件下的表达变化。结果表明,在盐和干旱胁迫条件下,盐芥根的伸长率和侧根数目显著高于拟南芥,表明前者抗逆性较强。ABA受体基因的表达在两者之间存在差异,PYL4、PYL5、PYL6、PYL7和PYL9在两者中表达变化趋势一致,PYR1、PYL1、PYL10和PYL13在盐芥和拟南芥中表达趋势相反,结合生理指标推测两者在ABA信号途径中可能存在差异。
Thellungiella halophila and Arabidopsis thaliana were adopted as materials in this study. The evolutionary relationship and conserved domains of ABA receptors were analyzed by bioinformatics tools. We compared the differences between T. halophila and Arabidopsis in root growth, RWC, osmotic pressure and the expression of ABA receptor genes under 400 mmol·L^-1 mannitol, 200 mmol·L^-1 Na Cl and 50 μmol·L^-1 ABA stress conditions, respectively. The results showed that T. halophila had higher root elongation and more lateral roots under salt and drought stress treatment, which revealed that T. halophila was more tolerant to abiotic stresses than Arabidopsis. Under the same treatment, the expression pattern of ABA receptors showed the difference between these two species. The expression of PYL4, PYL5, PYL6, PYL7 and PYL9 showed the same trend, while the expression of PYR1, PYL1, PYL10 and PYL13 had the opposite trend, which indicated that there may be some differences in ABA signaling pathway between T. halophila and Arabidopsis.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第8期1248-1256,共9页
Plant Physiology Journal
基金
山东省科技计划项目(2012GGB01136)
山东大学高校自主创新项目(201303050)
关键词
盐芥
拟南芥
ABA受体
盐胁迫
干旱胁迫
Thellungiella halophila
Arabidopsis thaliana
ABA receptors
salt stress
drought stress
