摘要
以羧甲基纤维素钠(CMC-Na)为惟一碳源对白蚁悬液进行富集培养,并从中分离菌株32株,通过纤维素刚果红初筛平板获得透明圈较大的8株菌,在此基础上进行摇瓶复筛,得到1株酶活较高的菌株(B3)。同时对其酶促反应温度、稳定性及其酶作用底物进行了测定。结果表明:CMCase的最适酶促反应温度为50℃,且在该温度下有较强的稳定性,保温30 min酶活基本保持不变,60 min酶活损失约14%,菌株B3所产纤维素酶对玉米秸秆纤维素粉有较强的水解能力,同时对滤纸和脱脂棉也有一定的降解能力。
Thirty-two strains were isolated from termite suspension with CMC-Na as the sole carbon source. We got eight colonies surrounded by some big transparent zone in cellulose-congo red agar medium. A strain （B3） of cellulose-decomposing strain was isolated by shake flask for the second screening. The optimal reacting temperature of cellulase secreted by strain B3 was 50 ℃. The specific enzyme activity was similar when temperature was kept at 50 ℃ for 30 min. However, for the case of 60 min, the enzyme activity lost about 14%. Under the same conditions, the straw enzyme activity was very high, and relative high FTA and absorbent cotton enzyme activity was observed.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第3期352-355,共4页
Journal of Food Science and Biotechnology
基金
国家973计划项目(2007CB714306)
关键词
白蚁
纤维素分解菌
酶活
酶学性质
termite
cellulose-decomposing microorganism
cellulase activity
enzyme property
