摘要
在 Ｓｈｉｍ－ｐａｃｋ ＣＬＣ－ＯＤＳ柱（５ｕｍ，４． ６ ｍｍｉ． Ｄ． × １５０ ｍｍ）上，研究了硫唑嘌呤及其中间体（６－巯基嘌呤、１－甲基－４－硝基－５－氯咪唑）的反相高效液相色谱分离检测的最适宜条件，流动相组成为乙腈－水（３０： ７０ Ｖ／Ｖ），流速为 ０． ８ ｍＬ／ｍｉｎ，检测波长为 ２１０ ｎｍ。经实际样品测定，结果满意。这为改进合成工艺、提高产品质量提供了一个快速有效的检测方法。
The RP-HPLC determination of Azathioprine and its intermediates (mercapum,4. 6 mm I. D-× 150 mm) column,acetonitrile-waters(30 : 70 V/V) as mobile phase flow rater, 0. 8 mL/min and UV 210 um detector. Good linearity was obtained in the concentration range of 0. 05-15 mg/L with standard calibration curve of Azathioprine y = 10982lx-2450,r = 0. 9999, Mercaptoprine y=68173x + 2034,r= 0. 9998, 1-methyl-4- nitro5-chlorine-imidazde y = 64438x-1353,r= 0. 9995 .The method has been used to determine drug samples with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第5期528-530,共3页
Chinese Journal of Analytical Chemistry
关键词
RP-HPLC
HPLC
中间体
测定
分析
硫唑嘌呤
Reversed phase high performance liquid chromatographiy, azathioprine,mercaptoprine, 1-methy l-4-nitro-5-chlorine-imidazde
