摘要
目的：建立以高效液相色谱法测定依达拉奉中苯肼残留量的方法。方法：色谱柱为Diamonsil C18，流动相为0．05mol·L^-1醋酸铵-乙腈（80：20），流速为1．0mL·min^-1，检测波长为233nm，进样量为20μL。结果：苯肼检测浓度的线性范围为22～550ng·mL^-1。（r=0．9999）；平均回收率为98．10％（RSD=0．85％），最小检测限为44Pg。结论：本方法简便可靠、灵敏度高，可用于该制剂中苯肼残留量的测定。
OBJECTIVE： To establish an HPLC method for the determination of phenylhydrazine residues in edaravone. METHODS： The determination was performed on Diamonsil C18 column with 0.05 mol·L^-1 ammonium acetate -acetonitrile （80 ： 20） as mobile phase at a flow of 1.0 mL · min^-1. The wavelength of UV detector was set at 233 nm and the sample size was 20 μL. RESULTS： The linear range of phenylhydrazine was 22- 550 ng·mL^-1（r = 0.999 9） ; The average recovery was 98.10% （RSD = 0.85%）, and the detection limit was 44 pg. CONCLUSION： The analytical method is simple, reliable and sensitive, and suitable for the determination of phenylhydrazine residues in edaravone preparation.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第31期2454-2455,共2页
China Pharmacy
