摘要
本文采用人羊膜基膜(HABM)做为腹腔巨噬细胞、CCLM24黑色素瘤细胞和神经细胞生长的支持物,MTT定量法测定这三种细胞在HABM上的生长。发现CCLM 24黑色素瘤细胞在HABM上生长最为活跃,神经细胞次之,而巨噬细胞生长受到抑制。HABM经肝素酶Ⅰ、Ⅱ消化后,CCLM 24细胞生长更加活跃。胶原酶和软骨素酶ABC消化后其生长活性降低25％和30％。神经细胞在肝素晦Ⅰ消化的HABM上生长活跃,而其余酶消化的HABM均使神经细胞生长活性降低。提示不同的细胞在HABM上的生长性质不同。
Human amniotic basement membrane (HABM) was used as a substratum for adult mouse peritoneal macrophages, CCLM24 melanoma cells, and neuronal cells from the forebrain of E8 chick embryo, in order to compare their cell attachment and growth properties. The number of attached cells on the HABM was counted microscopically. The extent of cell growth was quantified by a MTT automated colorimetric microassay measured by an Elisa reader. The counting results indicated that both neuronal and melanoma cells attach significantly better on the HABM than the plastic surface whereas the macrophages attach similarly on either the plastic surface or the HABM. However, the results for cell growth showed that the HABM inhibited 40% growth of macrophages, and that the HABM promoted the growth of melanoma and neuronal cells differently. Both heparinase Ⅰ- or Ⅱ- treated HABMs enhanced over 20% growth of melanoma cells compared to the native HABM. But, only the heparinase Ⅱ-treated HABM enhanced about 30% growth of neuronal cells, whereas the heparinase Ⅰ-, collagenase-, or chondroitinase ABCtreated HABMs did not support the attachment and growth of neuronal cells. However, both collagenase- or chondroitinase ABC-treated HABMs reduced 30% growth of melanoma cells. Therefore, it was concluded that the roles of HABM on the attachment and growth properties of these cells are different.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1989年第4期241-244,共4页
Chinese Journal of Anatomy
基金
香港大学
陈蕉琴和医学院研究基金
关键词
羊膜
人羊膜基膜
细胞生长
human amniotic basement membrane, CCLM 24 melanoma, macrophages, neuronal cells, enzymatic degradation, MTT automated colorimetric microassay.
