摘要
目的:了解珠海地区丙型肝炎患者的HCV基因分型情况,为丙型肝炎病毒的诊断和预防及治疗提供有力依据。方法:将53例HCV-RNA阳性的血清标本先逆转录为cDNA后,对其进行PCR扩增,然后采用反向点杂交技术(RDB)对扩增产物进行基因分型检测。结果:样本53例,共检出4种基因型,比例分别为1b型占60.38%,2 a型占7.55%,3b型占16.98%,6 a型占15.09%。结论:珠海地区HCV基因型以1b型为主,6 a型和3b型次之,2 a型比例相对较低,3b型高于全国其他地区。
Objective： To determine the HCV genotypes for the diagnosis of hepatitis C virus and provide a strong basis for prevention. Methods：53 cases of HCV - RNA - positive serum specimens for the first retrovirus eDNA, their PCR amplification, and then used Reverse dot blot （RDB） of the amplified product of a genetic sub - type testing. Results： The sample of 53 cases, four were detected genotype, respectively, lb -type accounting for 60. 38%, 2 a type of 7. 55%, 3 b -type accounting for 16. 98%, 6 a total of 15. 09%. Conclusion： In Zhuhai, HCV genotypes are 1 b - oriented, followed by 6 a - and - 3 b, a small 2 a type. 3b - higher than that of other parts of the country.
出处
《中国卫生检验杂志》
CAS
2009年第5期1071-1073,共3页
Chinese Journal of Health Laboratory Technology
基金
广东省科技局资助项目(PC20071002)
