摘要
目的:探讨菟丝子醇提液对衰老模型大鼠脾淋巴细胞DNA损伤的保护作用。方法:Wistar大鼠40只,按体重随机分为青年对照组(8只)、衰老模型组(8只)、菟丝子给药组(24只),菟丝子给药组又分为给药15d、30d和45d组。采用D-半乳糖法制作衰老模型,按0.8g/kg.d剂量经胃灌服菟丝子醇提液。采用单细胞凝胶电泳(SCGE)技术检测脾淋巴细胞DNA损伤程度,同时观察不同给药天数对其影响。结果:衰老模型大鼠脾淋巴细胞DNA拖尾率较青年组明显升高(P<0.01),不同菟丝子醇提液给药组均可降低大鼠脾淋巴细胞DNA拖尾率(P<0.01),以给药45d效果最显著(P<0.01)。结论:菟丝子醇提液对D-半乳糖衰老模型大鼠脾淋巴细胞DNA损伤具有保护作用,且呈现时间依赖性。
Objective： To study the protective action of alcoholic extract of semen cuscutae （AESC） upon DNA damage in in spleen lymphoeytes of aging model rats. Method：Forty Wistar rats were randomly divided into young（8） ,aging（8） ,and three different administration groups of 15d, 30 d and 45 d（24）. Aging rat models were made by D--gal injection,the AESC was drenched by a transgastric approach at the dosage of 0. 8g/kg.d. We ssayed the DNA damage in spleen tymphocytes of aging model rat by the single cell gel electrophoresis （SCGE） technique; at the same time, the effects of different days with AESC were observed. Results：Compared with the control group, the percentage of cells with comets of spleen in aging model rat increased （P 〈 0. 05） ; drenched by the AESC the percentage of cells with comets of spleen in aging model rat decreased （P 〈 0.05）. Conclusions：AESC can protect DNA damage of the spleen lymphocytes in aging rat induced by D -gal. And the protect Presents time dependence.
出处
《黑龙江医药科学》
2009年第3期2-2,3,共2页
Heilongjiang Medicine and Pharmacy
关键词
菟丝子醇提液
衰老模型大鼠
脾淋巴细胞
DNA损伤
alcoholic extract of semen cuscutae
aging model rats
spleen lymphocyte
DNA damage
