摘要
本文用免疫荧光双重标记技术对三叉神经尾侧亚核（Ｖｃ）和脊髓背角浅层内ＢａｎｄｅｉｒａｅａＳｉｍｐｌｉｃｉｆｏｌｉａＩｓｏｌｅｃｔｉｎＢ４（ＢＳＩ－Ｂ４）标记的Ｃ纤维及其终末与Ｐ物质（ＳＰ）能、降钙素基因相关肽（ＣＧＲＰ）能、谷氨酸（Ｇｌｕ）能和一氧化氮（ＮＯ）能纤维及其终末的分布和相互关系，三叉神经节（ＴＧ）和脊髓背根节（ＤＲＧ）中ＢＳＩ－Ｂ４标记并呈ＳＰ样、ＣＧＲＰ样、磷酸激活的谷氨酰胺酶（ＰＡＧ，谷氨酸能细胞的特异性标识物）样和ＮＯＳ样阳性的双标细胞进行了定位、定性和定量的系统研究。Ｖｃ和腰髓背角内ＳＰ样、ＣＧＲＰ样、ＰＡＧ样、ＮＯＳ样阳性纤维和终末及ＢＳＩ－Ｂ４标记的Ｃ纤维及其终末分布的主要重叠区域是Ⅱ层外带（Ⅱｏ）。ＴＧ和ＤＲＧ内可见ＢＳＩ－Ｂ４标记并呈ＳＰ样、ＣＧＲＰ样、ＰＡＧ样或ＮＯＳ样阳性的双标细胞，其中ＰＡＧ／ＢＳＩ－Ｂ４双标细胞的数量最多。此结果提示Ｃ纤维主要含Ｇｌｕ，Ｇｌｕ是初级传入最主要的神经活性物质。此外还有一些Ｃ纤维含ＳＰ、ＣＧＲＰ和ＮＯ。
Immunofluoresence histochemical double staining technique was used to qualitively examine the distributions and relationships between Bandeiraea Simplicifolia Isolectin B 4 (BSI B 4) labeled primary afferent C fibers and terminals and substance P (SP) , calcitonin gene related peptide (CGRP) , phosphate activated glutaminase (PAG, which is a specific marker for glutamatergic neurons) , nitric oxide synthase (NOS) like immunoreactive (LI) fibers and terminals in the superficial laminae of the trigeminal subnucleus caudalis (Vc) and dorsal horn of the spinal cord. SP/BSI B 4, CGRP/BSI B 4, PAG/BSI B 4, and NOS/BSI B 4 double labeled cells in the trigeminal ganglion (TG) and dorsal root ganglion (DRG) were also quantitatively examined. The mainly overlapping area of SP , CGRP , PAG , and NOS LI fibers and terminals and BSI B 4 labeled C fibers and terminals was in the outer part of laminae Ⅱ (Ⅱo). In the TG and DRG, some BSI B 4 labeled cells which give off C fibers also showed SP , CGRP , PAG , and NOS LI. Among those double labeled cells, the number of PAG/BSI B 4 double labeled cells was the most numerous. Present results indicate that most of the primary afferent C fibers and terminals contain glutamate (Glu) and Glu is the most important neural active substance for the primary afferent C fibers. Additionally, some primary afferent C fibers also contain SP, CGRP, and NO.
出处
《中国疼痛医学杂志》
CAS
CSCD
1998年第1期30-37,共8页
Chinese Journal of Pain Medicine
基金
国家自然科学基金
关键词
三叉神经
尾侧亚核
脊髓背角
C纤维
P物质
trigeminal subnucleus caudalis
dorsal horn of the spinal cord
C fiber
substance P
calcitonin gene related peptide
glutamate
nitric oxide
