摘要
目的:探讨人参总皂苷对肢体缺血再灌注骨骼肌的保护作用。方法:健康成年家兔20只,随机分为对照组和实验组,每组10只,建立兔肢体缺血再灌注损伤动物模型。在即将恢复血流灌注时,两组分别自耳缘静脉注射生理盐水(对照组)、人参总皂苷注射液(实验组)。分别在缺血前、缺血后2h、再灌注后1h、再灌注后3h采集术侧股静脉血样。测定血清乳酸脱氢酶(LDH)、肌酸激酶(CK)的含量。取上述各时相点动物胫前肌组织,测定骨骼肌组织湿/干重比值并观察肌组织微细和超微结构变化。结果:实验组再灌注后LDH、CK明显低于对照组(P<0.01),骨骼肌组织湿/干重比值低于同期对照组(P<0.05)。光镜及电镜下观察实验组骨骼机损伤轻于对照组。结论:人参总皂苷能够缓解组织水肿,保护缺血再灌注骨骼肌。
Objective：To study the protective effect and mechanism of Ginsenosides on ischemia repeffusion injury of skeletal muscle. Methods ：The experimental model of ischemia reperfusion injury in extremity were produced in 20 healthy adult rabbits which were randomly divided into experimental and control groups（each of 10 rabbits）. Normal saline injec- tion and Ginsenosides were infused intravenously into rabbits of control group,experimental group respectively just before blood reperfusion. At preischemia, 2 hours post - ischemia, l hour post - reperfusion and 3 hours post - reperfusion, sample of blood was collected from the femoral veins respectively for measurements of lactic dehydrogenase（ LDH）, creatine kinase（CK） by means of biochemical methods in every rabbit. Meanwhile the tibialis anterior muscles were harvested for the measurements of Wet/Dry ratio of skeletal muscle and for the microscope and ultrastructure examinations by using optical microscope and electronic microscope respectively. Results ： After reperfusion, the contents of LDH, CK were dramatically higher in control group （P 〈 0.01 ） but were not in experimental group（ P 〉 0.05）. The Wet/Dry ratio of skeletal muscle had not obvious difference in every group（ P 〉 0.05） before reperfusioin. The skeletal muscle injury of experi- mental groups, after reperfusion was of a mild degree than of control group in light microscope and electronic microscope examinations. Conclusion：Ginsenosides can effectively improve soft tissue swelling and protec skeletal muscle from ische- mia reperfusion injury.
出处
《中华中医药学刊》
CAS
2009年第5期1074-1076,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
人参总皂苷
肢体
再灌注损伤
ginsenosides
skeletal muscle
reperfusion injury
