摘要
本文介绍了植物细胞培养中胞内产物释放的研究现状,包括:1、化学法:(1)有机溶剂法,(2)抗生素法,(3)蛋白质、溶血卵磷脂、脱乙酰几丁质,(4)去污剂、螯合剂;2、物理法:(1)渗透压冲击,(2)温度冲击,(3)电处理,(4)超声波处理、激光打孔,3。
Progress in the release of plant cell intracellular products is discussed in this paper, including: 1.Chemical method: (1)Organic solvents, (2)Antibiotics; (3)Proteins, lysolecitinin, chitosan; (4)Detergents, chelating agent; 2. Physical methods: (1)Osmotic shock; (2)Temperature shock; (3)Electric treatment; (4)Ultrasonic treatment, laser technology; 3. pH perturbation and two mechanisms to alkaloid accumulation in plant cells.
出处
《生物工程进展》
CSCD
1998年第1期17-21,共5页
Progress in Biotechnology
关键词
植物细胞培养
次生代谢物
产物释放
细胞膜
,Plant Cell Culture
Secondary Products
Product Release
Cell Membrane