摘要
[目的]建立测定茄子叶中茄尼醇的RP-HPLC分析方法。[方法]采用Agilent 1100 HPLC，Hypersil ODS色谱柱（250mm×4．0mm，5mn），流动相y（甲醇）：y（乙醇）为60：40等度洗脱，洗脱流速1．0ml／min，柱温30℃，检测波长210am，保留时间约10．720min。[结果]茄尼醇质量浓度在6．90～220．80μg／m（R=0．9995）范围内线性关系良好，平均加样回收率（n=9）为99．94％，RSD=2．08％。通过与传统提取方法比较，确定最佳的提取方法为微波辐射法。[结论]所建方法具有简便、快速、准确、重复性好的特点，适用于茄子叶中茄尼醇的含量测定。
[Objective] The study aimed to establish a sensitive RP-HPLC method for determining the content of solanesol in eggplant leaves. [Method] The Agilent 1100 HPLC system, Agilent Hypersil ODS column （ A I. D. 250 mm × 4.0 mm, particle size 5 μm） was operated at a column temperature of 30 ℃ .The mobile phase was V （methanol） ： V（ethanol） = 60：40 at a flow rate of 1.0 mt/min.210 nm was set as the UV detection wavelength for solanesol, retention time was probable 10.720 min. [ Result ] The results showed a good linear relationship within 6.90- 220.80 pg/ml（ R = 0.999 5, the average recovery（ n = 9） is 99.94 %, RSD = 2.08 %. Comparison with conventional extraction method, the optimum extraction method was microwave irradiation. [ Conclusion] The method was rapid,simple,accurate and good reproducibility,it was suitable for the determination of solanesol of eggplant leaves.
出处
《安徽农业科学》
CAS
北大核心
2009年第12期5342-5343,5349,共3页
Journal of Anhui Agricultural Sciences
