摘要
花生致敏已成为重要的食品安全问题,花生蛋白中含有的Ara h 1是重要的致敏蛋白。运用生物信息学的电子克隆方法得到Shanyou523、Luhua14、USDA-Tifton三个花生品种的Ara h 1基因,并进行序列特征分析、多序列比对和结构域分析,探讨蛋白质水平的氨基酸差异与其致敏性的关系。
Peanut anaphylaxis has become an important food safety problem Peanut protein such as Ara h 1, is one of the major peanut allergens. Using In silico cloning method, we obtained the Ara h 1 genes from three species, Shanyou523, Luhual4 and USDA - Tifton, respectively, and then the characterization of sequences, the multiple sequence comparisons and the domain analyses were carried out to discuss the relationship between the diversities of the amino acids and anaphylaxis.
出处
《安徽科技学院学报》
2009年第2期31-37,共7页
Journal of Anhui Science and Technology University
基金
安徽省高等学校优秀青年人才基金重点项目(2009SQRZ112ZD)
安徽科技学院引进人才项目(ZRC2008215)
