摘要
用电子克隆方法获得甘蔗几丁质酶基因SCCHI1,采用生物信息学方法,对该基因编码蛋白从氨基酸组成、理化性质、跨膜结构域、疏水性/亲水性、亚细胞定位、高级结构及功能域等方面进行了预测和分析。结果表明:SCCHI1基因全长1236bp,包含一个完整的990bp的ORF,编码329个氨基酸。SCCHI1基因属于糖苷水解酶19家族,含有N-端信号肽、交连区、催化区,与高粱等其它植物的几丁质酶基因具有高度的相似性。为SCCHI1基因的分子克隆、功能鉴定和应用提供参考。
A novel chitinase gene SCCHI1 from sugarcane （Saccharum officinarum） was cloned in silico based on the corresponding sugarcane EST sequences in NCBI database.Some characters of amino acids encoded by SCCHI1 gene,including the composition of amino acid sequence,physical and chemical properties,transmembrane domain,hydrophobicity/hydrophilicity,subcellular localization,secondary and tertiary structure of protein plus the functional domain,were analyzed by bioinformatics tools.Bioinformatic analysis showed that the full-length SCCHI1 gene from sugarcane was 1 236 bp and it contained a complete ORF which encoded 329 amino acids.Furthermore,SCCHI1 belonged to glycoside hydrolase family 19 and contained a N-terminal signal peptide,a hinge region and a catalytic domain,which was highly conservative among chitinases from several different plant species.The results above provided some references for experimental cloning of SCCHI1 gene from sugarcane,identification and its application.
出处
《生物信息学》
2011年第4期322-326,330,共6页
Chinese Journal of Bioinformatics
基金
现代农业产业技术体系建设专项资金项目(CARS-20)
国家自然科学基金(30700514和31101196)
关键词
甘蔗
几丁质酶基因
电子克隆
生物信息学
Saccharum officinarum
Chitinase gene
In silico cloning
Bioinformatics
